Fig 1: Functional characterization of FNDC3B. (A) Representative images of IHC staining. Much stronger positive staining for FNDC3B was detected in HCC tissues than in adjacent non-tumor liver tissues. (B) Kaplan–Meier estimate of the length of survival for individuals with HCC, stratified by FNDC3B expression. Patients with lower FNDC3B expression in tumor tissues exhibited longer survival times. Scale bar represents 200 μm. IHC – immunohistochemistry; HCC – hepatocellular carcinoma.
Fig 2: Functional characterization of the rs2431 SNP. (A) Dual-luciferase reporter assays with the FNDC3B 3′UTR containing rs2431[G] or rs2431[A] in Hep3B (upper) and HEK293T (lower) cells. The regulatory activity of miR-409-3p on the 3′ UTR containing rs2431[G] was significantly weaker. (B) The suppression of FNDC3B expression in HCC cell lines containing the rs2431[A] allele was substantially stronger than that in HCC cell lines containing the rs2431[G] allele after miR-409-3p overexpression. (C) Representative images of IHC staining. Much stronger positive staining for FNDC3B was detected in HCC tissues with the rs2431[G] allele than with the rs2431[A] allele, and the signal was mainly detected in the cytoplasm. (D) A linear regression analysis showed a negative relationship between miR-409-3p and FNDC3B in HCC tissues with the rs2431[A] allele, but not in those with the rs2431[G] allele. Scale bar represents 200 μm. SNP – single nucleotide polymorphisms; IHC – immunohistochemistry; HCC – hepatocellular carcinoma.
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